abstract：:No major structural alteration of alkaline phosphatase can be observed in the early stages of enzyme oxidative inactivation by the ascorbate model system. Fluorescence changes of protein-bound 8-anilino-1-naphthalenesulfonic acid suggest, however, that localized modifications take place. Oxidized alkaline phosphatase ...

journal_title：Archives of biochemistry and biophysics

authors： Mordente A,Martorana GE,Miggiano GA,Meucci E,Santini SA,Castelli A

更新日期：1988-08-01 00:00:00

abstract：:Peribacteroid membranes can be isolated in essentially pure form from 20-day lupin root nodules by osmotic shock of the purified membrane enclosed bacteroids. The ATPase (EC 3.6.1.3) associated with this membrane has an acid pH optimum (5.25) and is specific for ATP (Mg-ATP Km = 0.16 mM). The enzyme activity requires ...

journal_title：Archives of biochemistry and biophysics

authors： Domigan NM,Farnden KJ,Robertson JG,Monk BC

更新日期：1988-08-01 00:00:00

abstract：:Phagocytes generate superoxide (O2-.) and hydrogen peroxide (H2O2) and their interaction in an iron-catalyzed reaction to form hydroxyl radicals (OH.) (Haber-Weiss reaction) has been proposed. Deferoxamine chelates iron in a catalytically inactive form, and thus inhibition by deferoxamine has been employed as evidence...

journal_title：Archives of biochemistry and biophysics

authors： Klebanoff SJ,Waltersdorph AM

更新日期：1988-08-01 00:00:00

abstract：:The steady-state kinetic mechanism of vitamin K-dependent carboxylase from calf liver has been investigated by initial-velocity measurements with varying concentrations of two carboxylase substrates and constant, nonsaturating concentrations of the other two substrates. With all combinations of the varied substrates t...

journal_title：Archives of biochemistry and biophysics

authors： Uotila L

更新日期：1988-07-01 00:00:00

abstract：:The cDNA probe H-9, originally constructed to recognize a portion of the mRNA for lapine synovial collagenase, also hybridized with a RNA of the same size (approximately 2.0 kb) isolated from activated lapine articular chondrocytes. Primary, monolayer cultures of lapine articular chondrocytes did not contain detectabl...

journal_title：Archives of biochemistry and biophysics

authors： Lin CW,Phillips SL,Brinckerhoff CE,Georgescu HI,Bandara G,Evans CH

更新日期：1988-07-01 00:00:00

abstract：:Affinity columns prepared by immobilizing monoclonal antibodies that specifically recognize the Lea or the Leb blood group antigens can be used for analytical or preparative isolation of oligosaccharides with the corresponding reactivities. The number of immobilized functional antibody combining sites on a column and ...

journal_title：Archives of biochemistry and biophysics

authors： Dakour J,Lundblad A,Zopf D

更新日期：1988-07-01 00:00:00

abstract：:The so-called 90,000-Da heat shock protein (hsp90) from chicken liver has been purified and physically characterized in the presence of high levels of the serine phosphatase inhibitor fluoride. The protein is an elongated dimer with a molecular weight of 160,000 and a frictional ratio of 1.6. On two-dimensional electr...

journal_title：Archives of biochemistry and biophysics

authors： Iannotti AM,Rabideau DA,Dougherty JJ

更新日期：1988-07-01 00:00:00

abstract：:Several experiments were carried out to study the difference between two isozymes (alpha(+) and alpha) of (Na+ + K+)-ATPase in the conformational equilibrium. Rat brain (Na+ + K+)-ATPase was much more thermolabile than the kidney enzyme. Both enzymes were protected from heat inactivation not only by Na+ and K+, but al...

journal_title：Archives of biochemistry and biophysics

authors： Matsuda T,Iwata H

更新日期：1988-06-01 00:00:00

abstract：:An alkaline proteinase, identical to mast cell chymase, has been described by a number of laboratories as being associated with myofibrils extracted from adult rat skeletal muscle tissue. A more recent study has indicated that chymase may be an intrinsic protein in the rat myocyte. The present study of rat myogenic ce...

journal_title：Archives of biochemistry and biophysics

authors： Hartz PA,Bird JW

更新日期：1988-06-01 00:00:00

abstract：:We tested the effects of a series of synthetic oligonucleotides (hybridons) complementary to the 5' noncoding and coding regions of rabbit beta-globin mRNA on endogenous protein synthesis in a rabbit reticulocyte cell-free translation system. With highly purified hybridons inhibition was completely specific for beta-g...

journal_title：Archives of biochemistry and biophysics

authors： Goodchild J,Carroll E 3rd,Greenberg JR

更新日期：1988-06-01 00:00:00

abstract：:Four different monoclonal antibodies (MAbs) to human epidermal growth factor (hEGF) were obtained from mouse hybridoma cell lines. They were highly specific for hEGF and did not cross-react appreciably with mouse EGF. The immunoglobulin class of these MAbs was IgG1 or IgG2a. The apparent Kd values for hEGF of these MA...

journal_title：Archives of biochemistry and biophysics

authors： Yoshitake Y,Nishikawa K

更新日期：1988-06-01 00:00:00

abstract：:The expression and activity of the phenobarbital (PB)-inducible P-450 isozymes, P-450b and P-450e, and the major 3-methylcholanthrene (MC)-inducible form, P-450c, were studied in primary cultures of adult rat hepatocytes in T1, Leibovitz L-15 (L-15), and a modification of Waymouth 752/1 (Way) media. P-450 isozymes in ...

journal_title：Archives of biochemistry and biophysics

authors： Turner NA,Wilson NM,Jefcoate CR,Pitot HC

更新日期：1988-05-15 00:00:00

abstract：:The influences of selenium deficiency (Se-D), chronic training, and an acute bout of exercise on hepatic and skeletal muscle antioxidant enzymes, i.e., superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPX), as well as glutathione S-transferase (GST) and tissue lipid peroxidation, were investigated in ...

journal_title：Archives of biochemistry and biophysics

authors： Ji LL,Stratman FW,Lardy HA

更新日期：1988-05-15 00:00:00

abstract：:Bovine kidney mitochondrial extracts contain an inactive protamine kinase and an inactive casein kinase. The protamine kinase was activated by chromatography on poly(L-lysine)-agarose. Two forms of this soluble mitochondrial protamine kinase were separated by chromatography on protamine-agarose. Both forms were purifi...

journal_title：Archives of biochemistry and biophysics

authors： Damuni Z,Reed LJ

更新日期：1988-05-01 00:00:00

abstract：:Chronic ethanol intoxication leads to the development of a resistance to lipid disordering by ethanol, a phenomenon known as "membrane tolerance". In the absence of the added ethanol, the lipid order, as measured by ESR and fluorescence techniques, does not necessarily change as a result of chronic ethanol ingestion (...

journal_title：Archives of biochemistry and biophysics

authors： Stubbs CD,Williams BW,Pryor CL,Rubin E

更新日期：1988-05-01 00:00:00

abstract：:Homogeneous preparations of fructose-1,6-bisphosphatase from mouse, man, rabbit, pig, and rat were tested as substrates for cyclic AMP-dependent protein kinase. Up to 1 mol of [32P]phosphate per mole enzyme subunit was incorporated into fructose-1,6-bisphosphatase from pig and rabbit liver, which should be compared wi...

journal_title：Archives of biochemistry and biophysics

authors： Ekdahl KN

更新日期：1988-04-01 00:00:00

abstract：:The nature of the purple complex formed upon the addition of octanoyl-CoA to the medium chain acyl-CoA dehydrogenase from pig kidney has been addressed by chemical quenching studies. Previous work, using quenching in 0.1 M KOH, suggested that the dehydrogenation product, trans-2-octenoyl-CoA, was not a participant in ...

journal_title：Archives of biochemistry and biophysics

authors： Lau SM,Thorpe C

更新日期：1988-04-01 00:00:00

abstract：:Three forms of 20-kDa myosin light chain (MLC), unphosphorylated, monophosphorylated, and diphosphorylated MLC (designated 20K, 20K-P, and 20K-PP) were demonstrated in thrombin-stimulated human platelets by two different gel electrophoretic methods: in the presence of glycerol urea or in two dimensions (isoelectric an...

journal_title：Archives of biochemistry and biophysics

authors： Naka M,Saitoh M,Hidaka H

更新日期：1988-03-01 00:00:00

abstract：:The crude extract prepared from Glycyrrhiza echinata cells treated with yeast extract catalyzed the formation of liquiritigenin (5-deoxyflavanone) and isoliquiritigenin (6'-deoxychalcone) in addition to naringenin (5-hydroxyflavanone) when incubated with 4-coumaroyl-CoA and malonyl-CoA in the presence of high concentr...

journal_title：Archives of biochemistry and biophysics

authors： Ayabe S,Udagawa A,Furuya T

更新日期：1988-03-01 00:00:00

abstract：:Purified human lecithin:cholesterol acyltransferase (LCAT) was covalently labeled by [3H]diisopropylflourophosphate with concomitant loss of enzymatic activity (M. Jauhiainen and P.J. Dolphin (1986) J. Biol. Chem. 261, 7023-7043). Some 60% of the enzyme was labeled in 1 h. Cyanogen bromide (CNBr) cleavage of the label...

journal_title：Archives of biochemistry and biophysics

authors： Farooqui JZ,Wohl RC,Kézdy FJ,Scanu AM

更新日期：1988-03-01 00:00:00

abstract：:Sendai virus envelopes have been a useful tool in studying the mechanism of membrane-membrane fusion and have served as a vehicle for introducing foreign molecules (e.g., membrane proteins) into recipient cells. Reconstituted Sendai virus envelopes are routinely obtained following solubilization of virus particles wit...

journal_title：Archives of biochemistry and biophysics

authors： Ran S,Nussbaum O,Loyter A,Marikovsky Y,Rivnay B

更新日期：1988-03-01 00:00:00

abstract：:Previous studies demonstrated that liver microsomes from untreated rats catalyze the omega, omega-1, and omega-2 hydroxylation of prostaglandins [K. A. Holm, R. J. Engell, and D. Kupfer (1985) Arch. Biochem. Biophys. 237, 477-489]. The current study examined the regioselectivity of hydroxylation of PGE1 and PGE2 by pu...

journal_title：Archives of biochemistry and biophysics

authors： Kupfer D,Jansson I,Favreau LV,Theoharides AD,Schenkman JB

更新日期：1988-02-15 00:00:00

abstract：:Previous studies in which dicyclohexylcarbodiimide (DCCD) was used to inactivate F1-ATPase enzymes have suggested that two glutamate residues in the beta-subunit are essential for catalysis. In the Escherichia coli F1-ATPase, these are residues beta-Glu-181 and beta-Glu-192. Oligonucleotide-directed mutagenesis was us...

journal_title：Archives of biochemistry and biophysics

authors： Parsonage D,Wilke-Mounts S,Senior AE

更新日期：1988-02-15 00:00:00

abstract：:The effects of alcohols on the reactivity of Azotobacter vinelandii hydrogenase were investigated. Hydrogenase catalyzed H2 oxidation coupled to methylene blue, benzyl viologen, or phenazine methosulfate when in the presence of solvents containing 15 or 40% ethanol or 40% methanol or 2-propanol. In general, the Km's f...

journal_title：Archives of biochemistry and biophysics

authors： Arp DJ

更新日期：1988-02-15 00:00:00

abstract：:The arachidonic acid epoxygenase metabolite 14,15-epoxyeicosatrienoic acid is further metabolized by rat liver microsomal fractions to regioisomeric diepoxides and epoxyalcohols. Diepoxides result from epoxidation at the 5,6-, 8,9-, or 11,12-olefins. Hydroxylation leading to epoxyalcohols with a cis, trans-conjugated ...

journal_title：Archives of biochemistry and biophysics

authors： Capdevila JH,Mosset P,Yadagiri P,Lumin S,Falck JR

更新日期：1988-02-15 00:00:00

abstract：:9-Hydrazinoacridine irreversibly labeled pig plasma amine oxidase by covalent attachment to the active carbonyl cofactor. The visible absorption spectrum of the modified protein displays new absorption bands at 495 and 525 nm. Its emission spectrum exhibited maxima at 415 and 440 nm. In addition, both absorption and e...

journal_title：Archives of biochemistry and biophysics

authors： Lamkin MS,Williams TJ,Falk MC

更新日期：1988-02-15 00:00:00

abstract：:All six available lysine residues in bacteriorhodopsin were amidinated with dimethyl-3,3'-dithiobispropionimidate, which is a crosslinking agent. The photocycle was studied by measuring light absorption and electric signals. The data show an essential change in the photocycle: instead of single components, the rise of...

journal_title：Archives of biochemistry and biophysics

authors： Tóth-Boconádi R,Taneva SG,Kiselev AV,Abdulaev NG,Keszthelyi L

更新日期：1988-02-01 00:00:00

abstract：:An amino acid-sensitive form of chorismate mutase (CM) has been purified over 1000-fold from disks excised from tubers of Solanum tuberosum L. cv White Rose. Purification was accomplished by chromatography on Matrix Blue A followed by affinity chromatography with tryptophan as ligand. CM assays performed in the absenc...

journal_title：Archives of biochemistry and biophysics

authors： Kuroki GW,Conn EE

更新日期：1988-02-01 00:00:00

abstract：:In this report we provide data, for the first time, demonstrating the conversion of the heme moiety of certain cytochrome P-450 and P-420 preparations, to biliverdin, catalyzed by heme oxygenase. We have used purified preparations of cytochromes P-450c, P-450b, P-450/P-420c, or P-450/P-420b as substrates in a heme oxy...

journal_title：Archives of biochemistry and biophysics

authors： Kutty RK,Daniel RF,Ryan DE,Levin W,Maines MD

更新日期：1988-02-01 00:00:00

abstract：:Limited proteolysis of the plasma membrane calcium transport ATPase (Ca2+-ATPase) from human erythrocytes by trypsin produces a calmodulin-like activation of its ATP hydrolytic activity and abolishes its calmodulin sensitivity. We now demonstrate a similar kind of activation of the human erythrocyte membrane Ca2+-ATPa...

journal_title：Archives of biochemistry and biophysics

authors： Wang KK,Villalobo A,Roufogalis BD

更新日期：1988-02-01 00:00:00

abstract：:Previous studies have indicated that activation of a normally unexpressed beta 1----3-N-acetylglucosaminyltransferase is responsible for the accumulation of a wide diversity of both type 1 and 2 lacto-series antigens in human colonic adenocarcinomas. A beta 1----3-N-acetylglucosaminyltransferase has been solubilized f...

journal_title：Archives of biochemistry and biophysics

authors： Holmes EH

更新日期：1988-01-01 00:00:00

abstract：:The mechanism of C4 acid decarboxylation was studied in bundle sheath cell strands from Urochloa panicoides, a phosphoenolpyruvate carboxykinase (PCK)-type C4 plant. Added malate was decarboxylated to give pyruvate and this activity was often increased by adding ADP. Added oxaloacetate or aspartate plus 2-oxoglutarate...

journal_title：Archives of biochemistry and biophysics

authors： Burnell JN,Hatch MD

更新日期：1988-01-01 00:00:00

abstract：:Synthetic peptides corresponding to the phosphorylation site in the myosin regulatory light chain from smooth muscle, Lys-Lys-Arg-Ala-Arg-Ala-Thr-Ser-Asn-Val-Phe-Ala ([Ala14,15]MLC(11-23] and containing a variety of hydroxyamino acid analogs at position 19, were tested as substrates for the smooth muscle myosin light ...

journal_title：Archives of biochemistry and biophysics

authors： Pearson RB,Floyd DM,Hunt JT,Lee VG,Kemp BE

更新日期：1988-01-01 00:00:00

abstract：:Mitochondrial carbonic anhydrase has previously been quantitated in liver mitochondria; it was not detected in guinea pig kidney cortical mitochondria. Evidence of this enzyme in rat kidney cortical mitochondria is reported. Electron microscopy showed that intact mitochondria were free of other intracellular organelle...

journal_title：Archives of biochemistry and biophysics

authors： Dodgson SJ,Contino LC

更新日期：1988-01-01 00:00:00

abstract：:Several 13C isotope effects of relevance to reactions involving carbamate and carbamoyl phosphate have been determined. The fractionation factor of carbamate relative to aqueous CO2 is 1.011; the equilibrium isotope effect on the reaction catalyzed by carbamate kinase is 0.9983. From these data we can calculate that t...

journal_title：Archives of biochemistry and biophysics

authors： Tipton PA,Cleland WW

更新日期：1988-01-01 00:00:00

abstract：:The specific tyrosine phosphorylation of glucose-6-phosphate dehydrogenase (G6PDH) by the epidermal growth factor (EGF) receptor in vitro is demonstrated. The Km values of the substrate G6PDH and of ATP for the receptor tyrosine kinase were ca. 1 and 10 microM, respectively. The rate of phosphorylation was EGF depende...

journal_title：Archives of biochemistry and biophysics

authors： Napier MA,Lipari MT,Courter RG,Cheng CH

更新日期：1987-12-01 00:00:00

abstract：:This study characterizes the structural and functional significance of sulfhydryl residues in human plasma heparin cofactor II (HCII). For quantification of sulfhydryl groups, the extinction coefficient of HCII was redetermined and found to be 0.593 ml mg-1 cm-1 using second-derivative spectroscopy and multicomponent ...

journal_title：Archives of biochemistry and biophysics

authors： Church FC,Meade JB,Pratt CW

更新日期：1987-12-01 00:00:00

abstract：:When applied in liquid paraffin to the upper surface of expanding spinach leaves, [1-14C]palmitic acid was efficiently and exclusively incorporated into the sn-1 position of cellular glycerolipids, principally phosphatidylcholine and triacylglycerol. A slow transfer of fatty acids from phosphatidylcholine to chloropla...

journal_title：Archives of biochemistry and biophysics

authors： Roughan PG,Thompson GA Jr,Cho SH

更新日期：1987-12-01 00:00:00

abstract：:The essential Zn(II) in bovine liver dihydropyrimidine amidohydrolase (DHPase) was removed by incubation with 2,6-dipicolinic acid and replaced with Mn(II). Electron paramagnetic resonance studies of Mn(II) binding show that there are four binding sites per tetramer, and the dissociation constant at pH 7.5 is 13.5 mic...

journal_title：Archives of biochemistry and biophysics

authors： Lee MH,Pettigrew DW,Sander EG,Nowak T

更新日期：1987-12-01 00:00:00

abstract：:The effects of orally administered secondary autoxidation products of linoleic acid in rat liver were investigated. Their administration led to two toxic effects on hepatic carbohydrate metabolism, as compared to the administration of saline or linoleic acid used as controls. One effect was depletion of glucose 6-phos...

journal_title：Archives of biochemistry and biophysics

authors： Ashida H,Kanazawa K,Minamoto S,Danno G,Natake M

更新日期：1987-11-15 00:00:00